Study on mitochondrial respiratory function in stored apheresis platelets using real-time energy metabolism detec-tion technology
Objective To explore the optimal parameters for detecting mitochondrial respiratory function(MRF)in a-pheresis platelets(AP)using real-time energy metabolism detection technology,and to study the change of MRF in AP dur-ing storage.Methods AP samples were collected and detected for MRF using the Seahorse XF device.Multiple parameters such as AP loading concentrations,washing conditions and mitochondrial respiratory regulatory drug concentrations were ad-justed.Finally,the MRF in AP stored for 1 and 5 days were studied.Results The optimal parameters for detecting MRF in AP were as follows:The loading concentration was 5×107/100 μL and basal respiration was 316±65;Basal respiration in washing group was 329±120 compared with 57±7 in unwashing group;The concentration of FCCP was 2 μmol/L.The basal respiration in AP for 1 day was 683±161,while was 140±23 for 5 days,P<0.05;The maximum respiration in AP for 1 day was 1 044±82,while was less than 200 for 5 days,P<0.05.There was a significant difference in MRF dynamic curves be-tween the two groups.Conclusion The real-time energy metabolism detection technology was able to detect MRF in AP ef-fectively.The MRF significantly damaged during AP storage,indicating that it may be one new mechanism of platelet storage lesion.
apheresis plateletsmitochondriarespiratory functionreal-time energy metabolism detectionstorage
万方数据
维普
