Objective To explore how to identify rare unexpected antibodies using routine serology methods in blood transfusion department of grassroots hospitals.Methods A patient with gastrointestinal bleeding with inconsistent forward and reverse blood typing,positive antibody screening and incompatible blood matching in our hospital was selected as the re-search object.Mixed human O cell absorption method was used for blood type identification,and 10-panel reagent red blood cells,manual polybrene test,2-mercaptoethanol(2-ME)test,blind match and pedigree study were used to explore the anti-body characteristics.Results The serological test results of the patient's serum were as follows:The blood group was type B and RhD positive,and negative for direct antiglobulin test;The antibody was IgM;The likelihood of this antibody being an-ti-s,anti-Fyaor anti-k was low,and compound antibodies could not be excluded;The blood of the patient did not match with blood samples of 20 blood donors in the major side,while it matched with all the blood samples of her three sisters.Conclu-sion When the antibodies of high-prevalence antigen series in plasma cannot be identified,the matched blood may be found tthrough family survey for irradiation and transfusion.
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