Objective In the process of proliferation and division,normal human cells reach the mortality stage M1 and mortality stage M2,which makes the cells stop division and apoptosis.This irreversible physiological process is also an in-herent anti-tumor mechanism.The limited ability of cell proliferation limits its role in basic research,clinical application,bioengineering and other fields.The development of immortalized cell lines with stable,continuous proliferation and normal structure and function has become a hot and difficult point in the research of cell biology.Immortalized cells are important sources for the production of engineered blood cells.This review discusses the molecular research process of immortalization technology which is widely used at present and describes the technology of immortalized cell de-immortalization.
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