High resolution allele frequency analysis of HLA-A,HLA-B,HLA-DRB1,HLA-C,HLA-DQB1 and HLA-DPB1 in Guangdong Cord Blood Bank
Objective To analyze the characteristics of HLA-A,HLA-B,HLA-DRB1,HLA-C,HLA-DQB1 and HLA-DPB1 allele polymorphisms among cord blood donors in Guangdong population. Methods According to HLA high resolu-tion genotyping data of 32717 samples of cord blood donors from Guangdong Cord Blood Bank from January 2009 to Decem-ber 2023,the allele frequencies were calculated by direct counting and the haplotype frequencies were calculated by using Arlequin software 3.5.2.2. Results A total of 102 HLA-A alleles,160 HLA-B alleles and 96 HLA-DRB1 alleles were de-tected in 32717 samples. Among them,46 HLA-DPB1 alleles were detected in 5377 samples,and 66 HLA-C alleles and 35 HLA-DQB1 alleles were detected in 13310 samples. The most common alleles were HLA-A*11:01 (28.96％),HLA-B*40:01 (15.23％),HLA-DRB1*09:01 (15.72％),HLA-C*01:02 (19.40％),HLA-DQB1*03:01 (20.85％) and HLA-DPB1*05:01 (40.79％). The most common 3 loci haplotype and 6 loci haplotype were HLA-A*02:07-B*46:01-DRB1*09:01 (1.55％),HLA-C*07:02-DQB1*03:01-DPB1*05:01 (1.77％),HLA-DRB1*09:01-DQB1*03:03-DPB1*05:01 (3.31％) and HLA-A*02:07-B*46:01-C*01:02-DRB1*09:01-DQB1*03:03-DPB1*05:01 (0.30％). Conclusion In this study,the allele and haplotype frequencies of HLA-A,HLA-B,HLA-DRB1,HLA-C,HLA-DQB1 and HLA-DPB1 were obtained in the cord blood donors in Guangdong,which can provide important reference data for HLA gene related research and the selection of donors for clinical application.
万方数据
维普
