摘要
目的 了解广东地区脐带血捐献人群HLA-A、HLA-B、HLA-DRB1、HLA-C、HLA-DQB1、HLA-DPB1等位基因多态性的分布特征.方法 根据2009年1月—2023年12月,32717份广东省脐带血造血干细胞库脐带血捐献者标本HLA高分辨基因分型数据,计算得到等位基因频率,使用Arlequin软件(3.5.2.2版本)估算单体型频率.结果 32717份标本中,共检出102个HLA-A、160个HLA-B、96个HLA-DRB1等位基因;其中的5377份标本检出46个HLA-DPB1等位基因,13310份标本检出66个HLA-C和35个HLA-DQB1等位基因.各位点频率最高的等位基因是:HLA-A*11:01(28.96％)、HLA-B*40:01(15.23％)、HLA-DRB1*09:01(15.72％)、HLA-C*01:02(19.40％)、HLA-DQB1*03:01(20.85％)、HLA-DPB1*05:01(40.79％).最常见的三位点、六位点单体型分别是:HLA-A*02:07-B*46:01-DRB1*09:01(1.55％)、HLA-C*07:02-DQB1*03:01-DPB1*05:01(1.77％)、HLA-DRB1*09:01-DQB1*03:03-DPB1*05:01(3.31％)、HLA-A*02:07-B*46:01-C*01:02-DRB1*09:01-DQB1*03:03-DPB1*05:01(0.30％).结论 本研究获得了广东地区脐带血捐献人群HLA-A、HLA-B、HLA-DRB1、HLA-C、HLA-DQB1、HLA-DPB16个位点基因频率和单体型数据,为HLA基因的相关研究和临床应用供者的选择提供了重要的参考数据.
Abstract
Objective To analyze the characteristics of HLA-A,HLA-B,HLA-DRB1,HLA-C,HLA-DQB1 and HLA-DPB1 allele polymorphisms among cord blood donors in Guangdong population. Methods According to HLA high resolu-tion genotyping data of 32717 samples of cord blood donors from Guangdong Cord Blood Bank from January 2009 to Decem-ber 2023,the allele frequencies were calculated by direct counting and the haplotype frequencies were calculated by using Arlequin software 3.5.2.2. Results A total of 102 HLA-A alleles,160 HLA-B alleles and 96 HLA-DRB1 alleles were de-tected in 32717 samples. Among them,46 HLA-DPB1 alleles were detected in 5377 samples,and 66 HLA-C alleles and 35 HLA-DQB1 alleles were detected in 13310 samples. The most common alleles were HLA-A*11:01 (28.96％),HLA-B*40:01 (15.23％),HLA-DRB1*09:01 (15.72％),HLA-C*01:02 (19.40％),HLA-DQB1*03:01 (20.85％) and HLA-DPB1*05:01 (40.79％). The most common 3 loci haplotype and 6 loci haplotype were HLA-A*02:07-B*46:01-DRB1*09:01 (1.55％),HLA-C*07:02-DQB1*03:01-DPB1*05:01 (1.77％),HLA-DRB1*09:01-DQB1*03:03-DPB1*05:01 (3.31％) and HLA-A*02:07-B*46:01-C*01:02-DRB1*09:01-DQB1*03:03-DPB1*05:01 (0.30％). Conclusion In this study,the allele and haplotype frequencies of HLA-A,HLA-B,HLA-DRB1,HLA-C,HLA-DQB1 and HLA-DPB1 were obtained in the cord blood donors in Guangdong,which can provide important reference data for HLA gene related research and the selection of donors for clinical application.
维普
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