实验用大林姬鼠种群微卫星标记开发及遗传特性分析
Development of microsatellite markers and analysis of genetic characteristics of laboratory Apodemus peninsulae population
张晴 1张曦文 1何松 1袁宝 1陈健 1任文陟 1权福实 1胡进平 1丁瑜1
作者信息
- 1. 吉林大学动物科学学院,长春 130062
- 折叠
摘要
目的 开发大林姬鼠多态性微卫星标记,丰富大林姬鼠遗传数据,为大林姬鼠遗传质量控制及基因定位等工作奠定基础.方法 基于大林姬鼠基因组序列筛选微卫星位点,挖掘微卫星引物,通过多重PCR技术分析群体的遗传多样性.结果 成功开发出 30 个微卫星标记,利用 60 份大林姬鼠基因组DNA对 30 个微卫星位点进行评价,共检测出 152 个等位基因,平均每个位点有 5.067 个等位基因;平均观察杂合度为 0.592;平均香农指数为 1.265;平均多态信息含量为 0.598.结论 基于本研究所开发的微卫星位点具有较好的多态性,能有效分析大林姬鼠群体的遗传多样性,适合为建立大林姬鼠遗传质量标准和遗传质量检测方法奠定基础.
Abstract
Objective Polymorphic microsatellite markers developed for Apodemus peninsulae can enrich its genetic data and lay a foundation for genetic quality control and gene mapping.Methods Microsatellite loci were screened based on the genome sequence of Apodemus peninsulae,and microsatellite primers were identified.The genetic diversity of the population was analyzed by multiplex PCR.Results Thirty microsatellite markers were successfully developed and evaluated using 60 samples of Apodemus peninsulae.A total of 152 alleles were detected,with an average of 5.067 alleles per locus.The average observed heterozygosity was 0.592.The average Shannon index was 1.265.The average polymorphism information content was 0.598.Conclusions Based on the microsatellite loci developed in this study,the genetic diversity of Apodemus peninsulae can be effectively analyzed,laying a foundation for establishing genetic quality standards and detection method.
关键词
大林姬鼠/微卫星位点/遗传多样性/封闭群Key words
Apodemus peninsulae/microsatellite loci/genetic diversity/closed colony引用本文复制引用
基金项目
国家重点研发计划(2022YFF0710503)
吉林省科技发展计划(20230505037ZP)
出版年
2024
国家科技期刊平台
NSTL
万方数据