非酒精性脂肪性肝病大鼠的血清溶血磷脂酰胆碱和氨基酸代谢轮廓研究
Metabolic profiles of serum lysophosphatidylcholine and amino acids in rats with nonalcoholic fatty liver disease
赵梅玉 1史欣悦 1周姝伶 1李海军 1樊淑婷 1熊印华1
作者信息
- 1. 江西科技师范大学药学院,南昌 330013
- 折叠
摘要
目的 利用代谢组学手段研究非酒精性脂肪性肝病(nonalcoholic fatty liver disease,NAFLD)大鼠血清的氨基酸和溶血磷脂酰胆碱(lysophosphatidylcholine,LPC)代谢轮廓,寻找可表征NAFLD的生物标志物并推测其发生的可能机制.方法 通过喂养高脂饮食饲料和腹腔注射CCl4 制备NAFLD大鼠,采用液相色谱-质谱联用技术测定对照组和NAFLD组的大鼠血清中15 种LPCs和18 种氨基酸的含量;采用主成分分析和正交偏最小二乘法-判别分析等方法分析大鼠NAFLD肝损伤后血清中LPC和氨基酸代谢轮廓的变化,运用Pearson相关分析方法分析生物标志物与NAFLD的相关性.结果 NAFLD组的大鼠血清LPC和氨基酸代谢轮廓明显偏离对照组,且能完全区分开;LPC(20∶1)、精氨酸和谷氨酸对NAFLD有显著贡献,这 3 个代谢物被鉴定为生物标志物;LPC(20∶1)和精氨酸与AST、ALT、LDL、TBIL 等血清生化指标显著相关.结论 血清溶血磷脂酰胆碱和氨基酸的代谢轮廓与NAFLD密切相关.
Abstract
Objective To use metabolomics method to study the metabolic profiles of amino acids and lysophosphatidylcholine(LPC)in the serum of rats with nonalcoholic fatty liver disease(NAFLD),to identify biomarkers for NAFLD,and to speculate on the possible mechanism responsible for its occurrence.Methods NAFLD rats were prepared by feeding a high-fat diet and intraperitoneal injection of carbon tetrachloride.Levels of 15 LPCs and 18 amino acids in the serum were determined in control and NAFLD rats by liquid chromatography-mass spectrometry.Changes in serum LPC and amino acid metabolic profiles in NAFLD rats were analyzed by principal component analysis and orthogonal partial least squares discriminant analysis.Correlations between biomarkers and NAFLD were analyzed by Pearson's correlation analysis.Results The metabolic profiles of serum LPC and amino acids differed significantly between the NAFLD group and the control group and were completely distinct.LPC(20∶1),arginine,and glutamic acid had significant contributions to NAFLD and were identified as biomarkers.Furthermore,LPC(20∶1)and arginine were significantly correlated with serum biochemical indicators such as aspartate transaminase,alanine transaminase,low-density lipoprotein,and total bilirubin.Conclusions The metabolic profiles of serum LPC and amino acids may be closely related to NALFD.
关键词
非酒精性脂肪性肝病/溶血磷脂酰胆碱/氨基酸/代谢轮廓Key words
nonalcoholic fatty liver disease/lysophosphatidylcholine/amino acids/metabolic profile引用本文复制引用
基金项目
江西省教育厅科学技术研究项目(GJJ2201313)
江西省高等学校精细化学品工程技术研究中心开放课题(JFCEC-KF-2202)
大学生创新创业训练计划(20231704202)
研究生创新专项(YC2023-S910)
出版年
2024
国家科技期刊平台
NSTL
万方数据