神经母细胞瘤肝及肾上腺移植模型比较
Comparison of liver and adrenal transplantation models of neuroblastoma
陈红霞 1谭志刚 1林惠然 2冯露平 1郑楚雅 1廖文峰 1曾如凤 1刘锦信 1卓振建1
作者信息
- 1. 北京大学深圳研究生院实验动物中心,广东深圳 518055;省部共建肿瘤化学基因组学国家重点实验室,广东深圳 518055
- 2. 中国科学院深圳先进技术研究院,广东深圳 518055
- 折叠
摘要
目的 建立神经母细胞瘤(neuroblastoma,NB)肝移植模型,并与肾上腺原位移植模型进行比较,探索模型的特征.方法 采用微量注射针将含有5× 105(SK-N-SH)细胞溶液沿肝叶长轴种植于小鼠肝左叶,造模结束后检测肿瘤的生长、转移情况、相关基因表达及肿瘤组织病理学变化.结果 小鼠接种肿瘤细胞21 d后成瘤率达100％,肿瘤的生长、转移、相关基因表达及病理特征明显.结论 本文通过更为简单的手术造模方法,成功构建了一种神经母细胞瘤肝移植模型,为NB研究者今后科学实验提供了更多理想的模型选择.
Abstract
Objective A neuroblastoma(NB)liver transplantation model was established and compared with the adrenal orthotopic transplantation model to explore its characteristics.Methods 5× 105 SK-N-SH cells were implanted along the long axis of the left lobe of mouse livers with a micro-injection needle.The growth,metastasis,expression of related genes,and histopathological changes of tumors were detected after the modeling.Results The tumor formation rate in mice inoculated with tumor cells reached 100％after 21 days,and tumor growth,metastasis,related gene expression changes,and pathological characteristics were apparent.Conclusions In this study,a neuroblastoma liver transplantation model was successfully constructed via a relatively simple surgical method to provide a more suitable choice for future scientific NB experiments.
关键词
神经母细胞瘤/肾上腺原位移植/肝移植/疾病模型/成瘤率Key words
neuroblastoma/orthotopic adrenal transplantation/liver transplantation/disease model/rate of tumor formation引用本文复制引用
基金项目
国家自然科学基金(82002636)
深圳市自然科学基金(JCYJ20220531093213030)
出版年
2024
国家科技期刊平台
NSTL
万方数据