摘要
目的 使用剖宫产净化(cesarean section purification)方法建立无菌金黄地鼠模型.方法 供胎母鼠选择SPF级金黄地鼠,雌雄按 1∶1 进行交配,见栓后与雄鼠分开饲养.代乳母鼠的合笼时间应早于供胎母鼠一周.怀孕的金黄地鼠临产时在超净工作台内进行子宫摘除术,传入净化用隔离包内,剥离子宫获得待净化乳鼠,分别使用无菌级ICR小鼠和无菌级SD大鼠进行代乳,成功离乳后转入饲养用隔离包内.每月对饲养用隔离包进行无菌状态检测.结果 共实施 3 次剖宫产手术,第 1 次和第 2 次代乳失败,第 3 次代乳成功,获得仔鼠 18 只,存活率 88%,离乳存活率 66%,依照GB/T 14926.41-2001 进行无菌金黄地鼠状态检测均为合格.结论 通过剖宫产净化技术,使用无菌级ICR小鼠和无菌级SD大鼠进行代乳,达到微生物净化目的,获得无菌化金黄地鼠模型.
Abstract
Objective A sterile golden hamster model was established by cesarean section purification.Methods SPF-grade donor female golden hamsters were selected,and males and females were mated 1∶1 and separated after mating.The cage time of the surrogate mothers was 1 week earlier than that of the donor mothers.Parturient golden hamsters underwent hysterectomy on a sterilized workbench,and the uteruses were transferred into isolation kits and stripped.To obtain sterile milk for milk replacement,sterile ICR mice and sterile SD rats were used.After successful separation,the hamsters were transferred to isolation kits to prepare for feeding.The sterility status of the feeding isolation kits was tested monthly.Results Three caesarean sections were performed,but the first and second lactations failed.The third milk replacement was successful,and 18 young hamsters were obtained with survival rates of 88%and 66%after weaning.All hamsters were quality tested by GB/T 14926.41-2001.Conclusions Using a cesarean section purification technique and sterile ICR mice and SD rats for microbial-free milk replacement,a sterile golden hamster model was obtained.
基金项目
中国医学科学院医学与健康科技创新工程项目(2022-12M-1-020)
维普
万方数据