Purification of Total Organic Acids from Chaenomeles speciosa and Study on Their Analgesic and Anti-inflammatory Effects
Objective: To select optimal anion exchange resins appropriate for separating and purifying total organic acids from the fruit of Chaenomeles speciosa and to investigate analgesic and anti-inflammatory effects of the total organic acids.Method: The yield of total organic acids was used for selecting the optimal anion exchange resins and concentration of HCl.The analgesic effect of the total organic acids and alcohol extract of C.speciosa were observed and compared by the hot-plate test and writhing analgesia test, and their anti-inflammatory functions were observed and compared by the method of xylene-induced ear edema in mice.Result: Amberliter IRA-400(CL) was optimal, and the optimal concentration of HCl was 0.3 mol·L-1.The purity of organic acids was 65.15％.The total organic acids of C.speciosa (0.15,0.3,0.6 g· kg-1 )had analgesic effect on the pain caused by hot and acetic acid, which could inhibit the inflammation induced by xylene and significantly reduce ear swelling of mice.Furthermore, there was no significant difference between the total organic acids and alcohol extract of C.speciosa (1.25,2.5,5 g·kg-1 ).Conclusion: The total organic acids of C.speciosa may be one of the effective components that have analgesic and anti-inflammatory effect.
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