首页|Folin-Ciocaileu比色法测定啤酒花中总多酚的含量

Folin-Ciocaileu比色法测定啤酒花中总多酚的含量

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目的:采用Folin-Ciocaileu比色法测定啤酒花中的总多酚的含量.方法:以没食子酸作为对照品,以福林试剂-碳酸钠溶液体系为显色剂,检测波长765 nm.结果:标准曲线的回归方程为Y=0.046 59+109.89X(r=0.999 4),线性范围0.049~0.595 g·L-1,稳定性、精密度、重现性、回收率的RSD 1.01%~1.53%,平均回收率分别为99.6%,101.7%,98.3%.结论:该方法简便、快速、准确,是检测啤酒花中总多酚成分的可靠方法,为控制啤酒花的内在质量提供了依据.
Determination of the Content of Total Polyphenol for Hops by Folin-Ciocaileu
Objective: To estabilsh an UV method for determination of the content of total polyphenol for hops.Method: Folin-Ciocaileu was adopted determining the content of total polyphenol by UV at 765 nm.Result:The total polyphenol was linear in the range of 0.049-0.595 g·L-1 with the relative standard deviation r = 0.999 4.The average recovery was 99.7% , 101.7% ,98.3%.Conclusion :The method is accurate and simple with good reproducibility, and can control the quailty of this preparation effectively.

何文静、张帆、田树革、王佳

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新疆名老中医特色方剂实验室,乌鲁木齐,830054

新疆医科大学中医学院,乌鲁木齐,830054

啤酒花 总多酚 FoLin-Ciocaileu比色法

国家自然科学基金

30801555

2011

中国实验方剂学杂志
中国中医科学院中药研究所 中国中西医结合学会中药专业委员会

中国实验方剂学杂志

CSTPCD北大核心
影响因子:1.62
ISSN:1005-9903
年,卷(期):2011.17(5)
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