金刚酒对肾阳虚大、小鼠模型的影响
Influence of Jingang Wine on Rat Model and Mouse Model with Deficiency of Kidney Yang
姜华 1王永洲 1胡君茹1
作者信息
- 1. 甘肃省中医药研究院中药研究所,兰州,730050
- 折叠
摘要
目的:观察金刚酒对大、小鼠肾阳虚模型补肾壮阳,提高机体性功能作用的影响.方法:60只雄性KM小鼠随机分为正常对照组、模型对照组、金匮肾气丸组、金刚酒组:高剂量、中剂量、低剂量.采用im 5%氢化可的松0.02 mL·g(-1),连续给药10d,制作肾阳虚模型,通过观察小鼠的体重、自主活动次数、低温游泳存活时间、前列腺与精液囊湿重、正常小鼠耐缺氧时间,以及观察60只去势雄性SD大鼠附性器官指标的变化,研究金刚酒的药效作用.结果:金刚酒不同剂量组对肾阳虚模型小鼠的体重增长率、前列腺与精液囊湿重、延长游泳时间和自主活动的总次数,与模型组比较差异有统计学意义(P<0.05).对正常小鼠的耐缺氧能力,与正常组比较差异有统计学意义(P<0.05).其中高剂量组的体重增长率最高为24.44%,自主活动次数高出模型组54次/min,小鼠的存活时间的高剂量组时间值与正常组值仅相差43.2 s.对去势雄性大鼠阴茎勃起时间,大鼠前列腺+精液囊和提肛肌附性器官指数,与模型组比较差异有统计学意义(P<0.05).结论:金刚酒具有补肾壮阳,改善性腺萎缩与生殖性功能的作用.
Abstract
Objective:To observe the influence of Jingang wine on the kidney yang and sexual function in rat model and mouse model with deficiency of kidney yang. Method: Rat model and mouse model of deficiency of kidney yang were made by intramuscular injection of hydrocortisone. The animal models were divided into control group, model group, positive group, and Jingang wine group (low doses, middle dose, and high dose, given 10 days). The pharmacological action of the wine was evaluated by body weight,autonomous activities, survival time of animal in hypothermia swimming, prostate weight and seminal vesicle weight, endurance time in hypooxygen, and sex organ accessories in male castrated rats. Result: Compared with the model group, there were obvious difference in the growth rate of weight, prostate weight and seminal vesicle weight, swimming time, and autonomous activities among different dose Jingang wine groups ( P ＜ 0.05 ). There was obvious difference in endurance time in hypooxygen condition compared with that of the control group ( P ＜ 0. 05 ). Compared with the model group, there were obvious differences in erection time and sex organ accessories in the castrated male rats, such as prostate and seminal vesicle and levator ani( P ＜ 0.05). Conclusion: Jingang wine would nourish the kidney and support yang, and could improve sexual atrophy and function.
关键词
金刚酒/肾阳虚证/补肾壮阳Key words
Jingang wine/syndrome of deficiency in kidney yang/nourishing kidney to support yang引用本文复制引用
出版年
2011
国家科技期刊平台
NSTL
维普
万方数据