首页|3种增溶性辅料致大鼠肝肾损伤

3种增溶性辅料致大鼠肝肾损伤

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目的:研究聚氧乙烯山梨糖醇酐单油酸酯(吐温80),solutol HS 15,聚氧乙烯(40)氢化蓖麻油3种辅料对大鼠肝、肾损伤作用.方法:吐温80,solutol HS 15,聚氧乙烯(40)氢化蓖麻油3种辅料分别按以下方式染毒.将大鼠72只按雌雄各半随机分为4组,每组6只,分别为生理盐水对照组、辅料低、中、高剂量组,按每只im 0.22 mL,每日注射1次,注射4周后,心脏取血后处死大鼠,称肝、肾质量计算脏器系数,测定大鼠血清中反映大鼠肝、肾功能的生化指标,并对肝、肾组织进行病理学观察.结果:吐温80 3个剂量组与对照组以及各剂量组之间对比无明显差异.solutol HS 15的高、中、低剂量组与对照组对比,AST,TP,ALP,ALB无明显差异,各剂量组之间对比,高剂量组与对照组、低剂量组与高剂量组的肝器系数存在显著性差异(P＜0.05)；高剂量组与对照组之间ALT存在显著性差异(P＜0.05)；低剂量组与高剂量组的ALB,TP,ALT存在显著性差(p＜0.05)；低、中剂量组之间TP存在显著性差异(P＜0.05)；高、中剂量组之间ALT存在显著性差异(P＜0.05).聚氧乙烯(40)氢化蓖麻油3个剂量组,AST,ALT,ALB,ALP,TP与对照组对比无明显差异,各剂量组之间对比,低、中剂量组之间、低、高剂量组之间,AST,ALT,ALB,ALP存在显著性差异(P＜0.05).光镜下3种辅料的剂量组与对照组对比,大鼠肝细胞、肾细胞除了炎症之外无明显病理改变.结论:通过3种辅料的比较,发现吐温80致大鼠肝、肾损伤较小.
Liver and Kidney Injury Induced by Three Excipients in Rats
Objective: To study liver and kidney injury induced by Tween 80, solutol HS 15 or polyoxyethylene (40) hydrogenated castor oil in rat. Method; The rats were randomly divided into such groups as control group, three dose groups of tween 80, three dose groups of solutol HS 15, three dose groups of polyoxyethylene (40) hydrogenated castor oil. Each rat was injected 0. 22 mL of corresponding excipients q. d.for 4 weeks.Serum biochemical indicators of rat liver, kidney function were detected. Weight of liver, kidney was weighed and organ coefficient was calculated. Liver and kidney pathological changes were observed by HE staining. Result:There was no significant difference between groups of Tween 80 and the control group. There was significant difference in liver and kidney function in different dose groups of solutol HS15 and difference dose groups of polyoxyethylene (40) hydrogenated castor oil ( P <0. 05 ) . There was no obvious pathological changes except some inflammatory cells in rat liver and kidney in rats in all the groups. Conclusion:Tween 80 might be the weakest one to cause liver and kidney injury in rats injected with the three kinds of excipients.

excipientstween 80solutol HS 15polyoxyethylene (40) hydrogenated castor oilliver injurykindney injury

张海燕、韦迎春、邬伟魁、杨军宣、杨明

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江西中医学院现代中药制剂教育部重点实验室,南昌 330004

西南交通大学材料先进技术教育部重点实验室,成都610003

成都中医药大学,成都611137

药用辅料 聚氧乙烯山梨糖醇酐单油酸酯 solutol HS 15 聚氧乙烯(40)氢化蓖麻油 肝损伤 肾损伤

中医药行业科研专项项目重t大新药创制重大专项中药新型给药系统技术平台"十一五"重大新药创制项目

2007080062009ZX09103-3932009ZX09310-005

2011

11-3495/R.20110823.1118.013

中国实验方剂学杂志
中国中医科学院中药研究所　中国中西医结合学会中药专业委员会

中国实验方剂学杂志

CSTPCD北大核心
影响因子：1.62
ISSN：1005-9903
年,卷(期)：2011.17(20)
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