Objective; To determine content of adenosine in Dioscorea opposita from the various cultivation areas. Method; A SunFire C]8 Colum (4.6 mm x250 mm,5 jjim) was used with Methanol- phosphate buffer(15: 85 ) as the mobile phase. The detective wavelength was set at 260 nm and the flow rate was 1. 0 mL ? Min "'. Result: Ten batches of the samples were analyzed. The content of adenosine ranged from 0.035 9-0. 104 0 mg ? G"1. Conclusion; The method was simple, reproducible and reliable. It can be used to control the quality of D. Opposita.