首页|基于炎症反应的香椿子总多酚抗大鼠心肌缺血再灌注损伤的机制研究

基于炎症反应的香椿子总多酚抗大鼠心肌缺血再灌注损伤的机制研究

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目的:观察香椿子总多酚对大鼠心肌缺血再灌注急性炎症的影响.方法:50只SD大鼠随机分成假手术组(冠脉下穿线不结扎+0.5％羧甲基纤维素钠)、模型组(冠脉结扎+0.5％羧甲基纤维素钠)、香椿子总多酚低剂量(XD,50 mg· kg-1+冠脉结扎)、中剂量(XZ,100 mg·kg-1+冠脉结扎)、高剂量(XG,200 mg· kg-1+冠脉结扎)组,各组均ig给药.采用左冠状动脉前降支结扎30 min再灌120 min的方法复制大鼠心肌缺血再灌注损伤模型.以ST抬高作为结扎成功的标志,以ST段逐渐回落、梗死性Q波出现作为再灌注成功的标志.再灌结束后行腹主动脉取血,分离血清,测定血清中白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)含量.取左心室缺血区组织10 mg用RT-PCR测定核因子kB (NF-kB)p65 mRNA的表达水平,其余心肌组织做病理切片,并在光镜下观察左心室心肌组织的形态学变化.结果:模型组大鼠血清IL-6含量为(638.88±188.94) ng·L-1,XD,XZ,XG组与模型组相比,血清IL-6含量显著下降,分别为(491.58±142.59),(306.85±80.60),(246.11 ±71.64) ng·L-1.模型组大鼠血清TNF-α含量为(216.23 ±58.67) ng·L-1,XD,XZ,XG组大鼠血清TNF-α水平与之相比显著降低,分别为(148.24 -±36.30),(157.62 ±52.06),(144.10 ±36.64) ng·L-1.模型组大鼠心肌组织中NF-kBp65/β-actin为0.61 ±0.05,香椿子总多酚给药组大鼠NF-kB p65/β-actin与之相比显著降低,分别为0.53 ±0.08,0.45 ±0.13,0.38 ±0.11.香椿子总多酚组与模型组相比,大鼠心肌细胞的形态学损伤较轻.结论:香椿子总多酚能够减轻大鼠心肌缺血再灌注急性炎症,对其产生一定的保护作用.
Mechanism of Total Polyphenols Extracted from Toona sinensis Roem on Acute Inflammation during Myocardial Ischemia-reperfusion in Rats
Objective: To investigate effects of total polyphenols extracted from Toona sinensis Roem on acute inflammation in injury induced by myocardial ischemia-reperfusion in rats. Method: Fifty SD rats were randomly divided into 5 groups: sham operation group, model group and total polyphenols extracted from T. sinensis groups in 3 doses. Myocardial ischemia/reperfusion injury in rats was induced by legating the left coronary artery (LAD) 30 min followed by a 120 min reperfusion, while rats in sham group were sutured only. Ligature success was indicated by elevation of segment, yet reperfusion success was indicated through gradual resolution of T wave with ST segment, and appearance of infract Q wave. After reperfusion, blood from abdominal aorta was collected, and serum were prepared. Then content of IL-6 and TNF-α in serum were detected. Finally, 10 mg myocardium tissue was cut from left ventricle ischemic area, and expression level of NF-κB p65 mRNA was tested. The rest myocardium tissues were prepared for pathological sections. Then the morphological changes in left ventricle ischemic area was observed through optical Microscope. Result: Content of IL-6 and TNF-a in model group serum were (638. 88 ± 188. 94), (216.23 ±58.67) ng·L-1 respectively. Compared with rats in model group, after administrated with polyphenols extracted from T. sinensis Roem, the content of IL-6 and TNF-a in serum was declined. NF-κB p65/β-actin in left ventricle ischemic area myocardium tissue of model group was 0. 61 ± 0. 05 , while myocardium tissue NF-κB p65/β-actin in total polyphenols extracted from T. sinensis groups were decreased significantly. Compared with model group, the morphological injury of cardiomyocytes in total polyphenols extracted from T. sinensis groups were alleviated significantly. Conclusion: Total polyphenols extracted from T. sinensis can play an intervention role on acute inflammation during myocardial ischemia-reperfusion in rats.

total polyphenols extracted from Toona sinensis Roemmyocardial ischemia-reperfusionacute inflammation

李红月、陈超

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湖北医药学院,湖北十堰442000

三峡大学医学院,湖北宜昌443002

香椿子总多酚 心肌缺血再灌注 急性炎症

2012

中国实验方剂学杂志
中国中医科学院中药研究所　中国中西医结合学会中药专业委员会

中国实验方剂学杂志

北大核心
影响因子：1.62
ISSN：1005-9903
年,卷(期)：2012.18(2)
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