Preparation Process of Dehydrodiisoeugenol in Myristica fragrans
Objective: To optimize the process of preparing dehydrodiisoeugenol chemical reference substance. Method: Taking extract rate of dehydrodiisoeugenol as index, the best extraction condition of dehydrodiisoeug-enol was determined by single factor test and orthogonal test L9 (34) , which was scaled up to pilot volume. Result: Raw material was extracted with 80% ethanol at a 10: 1 solid-liquid ratio for twice and 2 h each time, the yielded extract was first seperated by extraction with absolute ethyl alcohol、acetyl acetate. Afer this, by the methods of silicagel column chromatography and recrystallization we produce 9 g of reference substance was producued. Conclusion: The process is effective, simple and economic, and can meet thg need of gram-size preparation.
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