Objective: To establish a simple determination method of polysaccharide in Rhizoma Polygonatii, which is not influenced by the content of monosaccharide. Method: The total sugar was determined by phenol-sulfuric acid method while the concentration of reducing sugar was determined by 3, 5-dinitrosalicylic acid (DNS) method, the contentration of polysaccharide = Ct (the concentration of total sugar) - Cs ( the concentration of reducing sugar). Result: It was used to evaluate the content of polysaccharide collected in different area, the concentrations of polysaccharide in Polygonatum Rhizoma collected from Yunnan province are relatively higher. Conclusion: The results showed above methods are convenient, stable and accurate, which can be used for quality evaluation of Polygonatum Rhizoma and its preparations.
3, 5-dinitrosalicylic acid methodphenol-sulfuric acid methodPolygonatii Rhizomadetermination of polysaccharide
NETL
NSTL
万方数据
维普
