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两产地罗勒挥发油化学成分比较

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目的:比较分析广东广州与安徽毫州产罗勒挥发油化学成分差异.方法:用水蒸气蒸馏法提取罗勒挥发油,用气相-质谱联用仪(GC-MS)对挥发油成分进行分析,并通过峰面积归一化法计算各成分的相对百分含量;采用薄层色谱法进行比较分析.结果:广州产罗勒挥发油鉴定出29种化合物,并确定了其相对含量,占总含量的99.90%,主成分为对烯丙基茴香醚(83.082%)、芳樟醇(4.734%)、Τ-杜松醇(2.715%)、桉叶油素(2.251%).毫州产罗勒挥发油鉴定出31种化合物,并确定了其相对含量,占总含量的98.65%,主成分为芳樟醇(26.909%)、肉桂酸甲酯(21.85%)、T-杜松醇(17.411%)、喇叭茶醇(14.154%):薄层色谱显示广州产罗勒挥发油比毫州产者多了2个特征斑点.结论:产地对罗勒挥发油化学成分具有显著影响,气质联用和薄层色谱法均可用于罗勒挥发油的鉴别和产地分析,提示罗勒开发利用应明确品质产地.
Studies on Chemical Constituents of Volatile Oil of Ocimum basilicum from Two Different Sources
Objective; To analyze the chemical constituents of volatile oil from Ocimum basilicum collected in two different sources. Method: The volatile components were extracted by steam distillation method, and analyzed by GC-MS. Peak area normalization method was used for the relative content of volatile oil. TLC was used for the identification of two samples. Result; Twenty-nine components were identified in 0. basilicum from Guangzhou, which accounted for 99.90% of the total relative content. The main components were p-allylanisole (83.082%), linalool 4. 734% ), tau-cadinol (2.715%), eucalyptol (2.251%). Thirty-one components were identified in 0. basilicum from Bozhou, which accounted for 98.65% of the total relative content. The main components were linalool (26.91%), methyl cinnamate (21.85%), tau-cadinol ( 17.41%), ledol (14. 154% ). Two characteristic spots could not be identified in 0. basilicum. from Bozhou but from Guangzhou. Conclusion: The volatile oil from different sources had obviously different chemical constituents. GC-MS and TLC can be used for the identification and analysis of sources. It pointed out that 0. basilicum should clear about its quality and sources before exploitation and application.

Ocimum basilicumvolatile oilGC-MSTLC

袁旭江、林励、谭翠明

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广州中医药大学中药学院,广州 510006

广东药学院,广州 510006

罗勒 挥发油 气质联用 薄层色谱法

2012

中国实验方剂学杂志
中国中医科学院中药研究所 中国中西医结合学会中药专业委员会

中国实验方剂学杂志

北大核心
影响因子:1.62
ISSN:1005-9903
年,卷(期):2012.18(11)
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