The in vitro Effects of Huanglian Jiedu Decoction against Pseudomonas aeruginosa Biofilms and its Synergism with Azithromycin on Planktonic Pseudomonas aeruginosa
Objective; To investigate the effects of Huanglian Jiedu decoction against Pseudomonas aeruginosa biofilms and its synergistie antibacterial in combination with azithromycin. Method; The microdilution assay was used to detect MIC of Huanglian Jiedu decoction against P. aeruginosa, checkerboard method was used to examine synersism of Huanglian Jiedu decoction and azithromycin. MTT assay was used to detect SMIC of Huanglian Jiedu decoction against P. aeruginosa biofilms, and microscopes was used to observe morphology of biofilms treated with the two drugs. Result; MIC of Huanglian Jiedu decoction against P. aeruginosa was 100 g-L-1 and azithromycin 200 mg·L-1. After the combination of two-drug MIC was 25 g·L-1 and 25 mg· L-1 fractional inhibitory concentration ( FIC) 0. 1875, which showed a synergismwith azithromycin. SMIC30of Huanglian Jiedu Decoction against P, aeruginosa biofilms at 1, 3, 7 d was 15.6, 15.6, 31.25 g·L-1, respectively; SMIC80 of the biofilms was 250, 250, 250 g ·L-1 , respectively. Morphologically Huanglian Jiedu decoction inhibited P. aeruginosa biofilms evidently at the concentration of SMIC80. Conclusion; Huanglian Jiedu decoction could inhibit P. aeruginosa biofilms, and has a synergism with azithromycin.
NETL
NSTL
万方数据
维普
