Effect of Total Saponin of Dioscoreae Nipponicae Rhizoma on PI3K/AKT Signal Pathway by in rIL-1β Induced Fibroblast-Like Synoviocytes
Objective; To explore the effects of total saponin of Dioscoreae Nipponicae Rhizoma (RDN) on in vitro apoptosis of rIL-1β induced fibroblast-like synoviocytes (FLS) , and further to investigate the PI3K/ AKT signal mechanism. Method; FLS were primarily cultured. Model group; 10 μg -L~ rIL-1β, RDN group; 10 μg -L-1 rIL-1βand 100 μg -L-1 RDN. Western blot method was applied to examine the expression of p-PI3K protein and p-AKT protein degradation in rIL-ly3 induced FLS stimulated with total saponin of RDN. Result; Compared to control group, expression of p-PI3K and p-AKT protein increased in model group, respectively 162. 66 ±2. 34 and 157. 03 ± 1. 84 (P <0. 05) , total saponin of RDN inhibited rIL-1β induced phosphorylation of AKT and PI3K in FLS, respectively 67. 84 ± 1. 24 and 71. 66 ± 1. 20 (P < 0. 01). Conclusion: Total saponin of RDN can inhibit FLS PI3K/AKT signal pathway. Total saponin of RDN may provide a new therapeutic approach in treatment of gouty arthritis.
NETL
NSTL
万方数据
维普
