Optimization of Water Extraction Technology for Pheretima aspergillum by Orthogonal Test
Objective; To optimize water extraction technology conditions of Pheretimaaspergillum. Method: With the content of hypoxanthine as index, based on single factor test, effects of extraction time, extraction times and the amount of water were investigated by L9 (34) orthogonal test. HPLC was used to determine the content of hypoxanthine. Result; Influencing order of various factors on water extraction technology was extraction times > extraction time > the amount of water; Optimal water extraction technology was as following: reflux extracted 1 time with 10 times the amount of water, extraction time of one hour. Conclusion; This optimized technology was stable and feasible.
Pheretima aspergillumextraction technologysingle factor testorthogonal test