Determination of Content Change of Two Lactone Ingredients from Andrographis Herba after Compatibility with Artemisiae Scopariae Herba by HPLC
Objective:To investigate effects of Andrographis Herha compatibility with Artemisiae Scopariae Herba on content change of andrographolide and dehydroandrographolide.Method:RP-HPLC was performed on an Innersil ODS-3 column (4.6 mm ×250 mm,5.0 μm) with mobile phase of acetonitrile-water in gradient elution,flow rate was 1.2 mL · min-1,detection wavelengths were set at 225 nm for andrographolide in 0-10 min and 254 nm for dehydroandrographolide in 10-20 min,column temperature was 35 ℃.Result:The linear range of andrographolide and dehydroandrographolide were 19.66-314.6 (r =0.999 5),4.3-69.12 g· L-1 (r =0.999 5),respectively.Average recoveries of these two components were 99.7％ and 99.9％,respectively with RSD were less than 2.3％.Conclusion:The contents of andrographolide and dehydroandrographolide from Andrographis Herba increased significantly after compatibility with Artemisiae Scopariae Herba.
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万方数据
维普
