HPLC测定密蒙花配方颗粒中蒙花苷含量
Determination of Buddleoside in Buddlejae Flos Formula Granules by HPLC
唐维宏 1韦建华 2马云婷2
作者信息
- 1. 北海市中医医院,广西北海 536000
- 2. 广西中医药大学,南宁 530001
- 折叠
摘要
目的:建立密蒙花配方颗粒中蒙花苷的含量测定方法.方法:采用Inertsil ODS3 C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.1％磷酸(55∶45),流速1.0 mL· min-1,柱温20℃,检测波长327 nm,进样量10 μL.结果:蒙花苷进样量在0.104 8 ～1.048 μg呈良好线性关系(r=0.9996),平均加样回收率102.2％(RSD 1.9％).结论:该方法操作简便、结果准确、重复性良好,可作为密蒙花配方颗粒中蒙花苷的含量测定方法.
Abstract
Objective:To establish a method for determining buddleoside in Buddlejae Flos formula granules.Method:Buddleoside was separated on Inertsil ODS3C18 column (4.6 mm × 250 mm,5 μm) with methanol-0.1％ phosphoric acid (55∶ 45) as mobile phase,flow rate was 1.0 mL ·min-1,column temperature was at 20 ℃,detection wavelength was 327 nm,injection volume was 10 μL.Result:Buddleoside had good linear relationship within the range of 0.104 8-1.048 μg with r =0.9996,the average recovery was 102.2％ with RSD 1.9％.Conclusion:This method was simple,accurate and reproducible,it could be used to determine the content of buddleoside in Buddlejae Flos formula granules.
关键词
密蒙花/配方颗粒/蒙花苷/高效液相色谱法Key words
Buddlejae Flos/formula granules/buddleoside/HPLC引用本文复制引用
出版年
2013
国家科技期刊平台
NSTL
维普
万方数据