SPME-GC-MS法研究竹叶柴胡和北柴胡挥发性成分差异
Different Analysis of Essential Oil from Bupleurum marginatum and Bupleurum chinese by SPME-GC-MS
王砚 1王书林2
作者信息
- 1. 成都中医药大学药学院,成都611137;四川省食品药品检验检测院,成都 611731
- 2. 成都中医药大学药学院,成都611137
- 折叠
摘要
目的:对竹叶柴胡和北柴胡进行挥发性成分的比较和分析,指出竹叶柴胡和北柴胡的差异性和共同性.方法:利用固相微萃取技术(SPME)提取,采用气质联用(GC-MS)进行分离和鉴定.结果:共鉴定出竹叶柴胡48个成分,北柴胡21个成分,其中有10个化合物为共有峰,相对质量分数最高的均为n-十六烷酸.结论:SPME-GC-MS能全面快速分析柴胡挥发油成分,并作为柴胡药材质量评估和基源鉴定的科学手段.
Abstract
Objective:To find the difference and the identity through analyzing the essential oil of Bupleurum marginatum and B.chinese.Method:The essential oil components of B.marginatum and B.chinese were extracted by solid phase micro extraction (SPME) and analyzed by GC-MS.Result:Forty-eight compounds were identified from B.marginatum.and 21 from B.Chinese.Ten common compounds were found and n-hexadecanoic acid was the main compound of highest relative content in both of B.marginatum and B.chinense.Conclusion:The information about the constituents of essential oil can be obtained entirely and quickly by SPME-GC-MS which is the scientific method of quality assessment and origin identification.
关键词
竹叶柴胡/北柴胡/挥发油/固相微萃取-气质联用/n-十六烷酸Key words
Bupleurum marginatum/Bupleurum chinese/essential oil/SPME-GD-MS/n-hexadecanoic acid引用本文复制引用
基金项目
国家星火计划项目(2010GA810056)
出版年
2014
国家科技期刊平台
NSTL
维普
万方数据