金花茶不同部位多糖的测定及体外抗氧化活性
Determination Polysaccharides and Antioxidant Activity of Different Parts of Camellia chrysantha
牛广俊 1朱思 1陈清英 1陈天增 2陈学圣 2梁一池1
作者信息
- 1. 福建中医药大学药学院,福州 350122
- 2. 漳平市展宏金花茶专业合作社,福建漳平 364400
- 折叠
摘要
目的:测定金花茶不同部位的多糖含量并探讨其体外抗氧化活性的差异.方法:运用超声提取方法提取金花茶叶子、芽尖、果壳、花的多糖,采用苯酚-硫酸比色法测定多糖的含量,通过2,2'-联氮-(3-乙基苯并噻唑-6-磺酸)二铵盐(ABTS+)法、1,1-二苯基-2-三硝基苯肼(DPPH)检测法、邻苯三酚法和邻二氮菲法检测金花茶叶子、芽尖、果壳和花水提物的抗氧化能力,应用综合评分法,对各水提物的体外抗氧化活性进行综合评价.结果:金花茶花、叶、芽尖、果壳中多糖含量分别为32.88,29.48,35.89,30.02 g·kg-;清除ABTS+自由基能力抗坏血酸(0.100 g·L-1)＞花(0.135 g·L-1)＞果壳(0.165 g·L-1)＞芽尖(0.243 g·L-1)＞叶(0.330 g·L-1);清除DPPH自由基能力顺序为抗坏血酸(0.200 g·L-1)＞花(0.344 g·L-1)＞果壳(0.435 g·L-1)＞芽尖(0.881 g·L-1)＞叶(1.011 g·L-1);超氧阴离子自由基抑制率抗坏血酸(0.2 g·L-1)≥芽尖(25.0 g·L-1)＞果壳(25.0 g·L-1)＞花(25.0 g·L-1)＞叶(25.0 g·L-1);当生药浓度达到25.0 g·L-1时,花、芽尖、果壳对羟基自由基消除率均大于50％,但均小于抗坏血酸(2 g·L-1);综合评分为芽尖(55.05)＞花(52.79)＞果壳(51.97)＞叶(23.73).结论:苯酚-硫酸比色法测定金花茶不同部位的多糖含量稳定可行,金花茶水提物具有一定抗氧化能力且不同部位抗氧化能力存在明显的差异.
Abstract
Objective:To measure polysaccharide content in different parts of Camellia chrysanthaand explore the differences in antioxidant activity in vitro.Method:Extract polysaccharides from different parts of C.chrysantha by ultrasonic extraction,phenol-sulfuric acid method was used to determine polysaccharides content and measure antioxidant capacity in different parts of Camellia water extract by ABTS method,DPPH assay,pyrogallol method and phenanthroline assay.Result:C.chrysantha flowers,leaves,bud tip,husk polysaccharide contents were 32.88,29.48,35.89,30.02 g ·kg-1.The ability to clear ABTS+ ·order was ascorbic acid (0.100 g ·L-1) flowers (0.135 g·L-1) ＞nutshell (0.165 g·L-1) ＞bud tip (0.243 g·L-1) ＞leaf (0.330 g·L-1),the ability to clear DPPH order was Ascorbic acid (0.200 g ·L-1) ＞ flowers (0.344 g ·L-1) ＞ nutshell (0.435 g · L-1) ＞bud tip (0.881 g·L-1) ＞leaf(1.011 g· L-1).The ability of clear Superoxide anion radical order was Ascorbic acid (0.2 g·L-1) ≥ bud tip (25.0 g·L-1) ＞nutshell (25.0 g·L-1) ＞flowers (25.0 g·L-1) ＞ leaves (25.0 g ·L-1).When crude drug concentration was 25 g ·L-1,the flowers,the cusp,nutshell elimination rate of hydroxyl radicals were greater than 50％,however all were less than ascorbic acid (0.2 g · L-1).The comprehensive score of bud tip (55.05) ＞ flowers (52.79) ＞ nutshell (51.97) ＞ leaf (23.73).Conclusion:Phenol-sulfuric acid colorimetry polysaccharide content in different parts of C.chrysantha was viable and stable and aqueous extract of C.chrysantha has a strong antioxidant capacity,the antioxidant capacity are significant differences and depend on different parts of C.chrysantha.
关键词
金花茶/不同部位/多糖/含量测定/抗氧化活性Key words
Camellia chrysantha/different fractions/polysaccharides/content determine/antioxidant activity引用本文复制引用
基金项目
福建省科技厅重点项目(2011N1007)
福建省龙岩市资助项目(201367)
出版年
2014
NETL
NSTL
维普
万方数据