Effects of Total Flavonoe of Epimedium of Two Species in Guizhou on IR-related Factors in Type 2 Diabetes
Objective:To study the effects of total flavonoe of Epimedium (TFE) from Epimedium acuminatum and E.Leptorrhizum grown in Guizhou on insulin resistance (IR)-related factors in mice induced by alloxan (ALX).Method:Hundred male mice were divided into normal group and model group.The mice in model group were fed with high-fat diet for 6 weeks,then single intraperitoneal injection of 2％ ALX solution (200 mg ·kg-1) was made to establish type 2 diabetes models.The modeling mice were randomly divided into 6 groups with 10 in each group,including metformin group (150 mg ·kg-1),E.acuminatum TFE group (100,50 mg· kg-1),E.leptorrhizum TFE group (100,50 mg ·kg-1),respectively,once a day for 14 days.After 14 days,the animals were fasted for 12 hours,and killed.Then the level of serum insulin (FINS) and C peptide was determined.The blood sugar (FBG) and BG after injection of ins 40 min were detected.Insulin tolerance (ITT),insulin resistance index (HOMA-IR) and pancreatic index were calculated.HE staining was used to observe the morphologic changes of pancreas in mice.Result:Compared with the normal group,blood sugar of the model group increased significantly (P ＜ 0.01),C-peptide and FINS significantly increased (P ＜ 0.05),pancreatic tissue injury was serious (P ＜ 0.01),the pancreas index decreased significantly (P ＜ 0.01).Compared with model group,two TFE from E.acuminatum all obviously improved ITT (P ＜ 0.05),pancreatic index (P ＜ 0.05),reduced C-peptide,FINS and HOMA-IR (P ＜ 0.05) and obviously alleviated pancreatic tissue injury (P ＜0.05) in mice induced by ALX.TFE from E.acuminatum had more obvious effect (P ＜0.05,P ＜0.01).Conclusion:Two kinds of TFE from E.acuminatum have certain effect on the type 2 diabetes mice induced by ALX,and can increase insulin sensitivity,improve IR-related factors on the type 2 diabetes mice,and have a good protective effect on the pancreatic tissue injured.Especially,TFE from E.acuminatum has obvious effect.Epimedium's active ingredient on diabetes may has a greater relationship with icariin.
Epimedium acuminatumEpimedium leptorrhizumtotal flavonoe of Epimediumalloxantype 2 diabetesinsulin resistance
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万方数据
维普
