首页|复方调元散挥发性成分分析

复方调元散挥发性成分分析

扫码查看
目的:研究复方调元散中的挥发性成分,探讨其治疗自闭症及相关症状的药效物质基础.方法:分别采用顶空固相微萃取法(HS-SPME)、水蒸气蒸馏法(SD)结合气质联用技术(GC-MS)对复方中挥发性成分进行分析鉴定;采用HS-SPME-GC-MS法对煎煮前后复方调元散挥发性成分进行对比分析.结果:SD提取的复方挥发油中共检测出48种挥发性成分,鉴定出28种,占挥发性成分总量的85.44%;HS-SPME从煎煮前复方粉末中检测出挥发性成分122种,鉴定出73种,占挥发性成分总量的75.56%,从煎煮后复方浸膏中检测出挥发性成分50种,鉴定出27种,占挥发性成分总量的93.49%.3次不同试验共鉴定出复方挥发性成分97种,含量最高的为α-细辛脑.结论:该研究为复方调元散的挥发性成分分析提供了方法学基础,同时也为其治疗自闭症药效物质基础研究提供了参考依据.
Analysis of Volatile Components of Tiaoyuan Powder
Objective:Study the volatile components of Tiaoyuan powder and explore its pharmacodynamic material basis for the treatments of autism and related symptoms.Method:Headspace solid phase micro-extraction (HS-SPME) and steam distillation (SD) combined with gas chromatography and mass spectrometry technique (GC-MS) were used to analyze and identify volatile components;HS-SPME-GC-MS were used to analyze volatile components before and after decoction.Result:48 volatile components were detected by SD-GC-MS,among them 28 were identified,accounting for 85.44% of the total volatile components.122 volatile components were detected before the decoction,73 were identified,accounting for 75.56% of the total volatile components.50 volatile components were detected after the decoction,27 were identified,accounting for 93.49% of the total volatile components.In three experiments a total of 97 peaks were identified;the highest content was α-asarone.Conclusion:This study not only provided a methodological foundation for volatile components analysis of Tiaoyuan powder,but also provided a reference on phamacodynamic material basis for improving autism.

Tiaoyuan powdervolatile componentsautismHS-SPMEsteam distillation

许俊洁、卢金清、万丽娟、林杰、屠寒、黎强

展开 >

湖北中医药大学,湖北省药用植物研发中心,武汉 430065

调元散 挥发性成分 自闭症 顶空固相微萃取 水蒸气蒸馏法

湖北省自然科学基金

2004AB242

2015

中国实验方剂学杂志
中国中医科学院中药研究所 中国中西医结合学会中药专业委员会

中国实验方剂学杂志

CSTPCDCSCD北大核心
影响因子:1.62
ISSN:1005-9903
年,卷(期):2015.21(11)
  • 4
  • 8