Dynamic Changes of Chemical Compositions in Different Organs of Cultivated Arctium lappa from Ningxia During Different Growth Periods
Objective: To study the dynamic changes of chemical compositions in different organs of cultivated Arctium lappa from Ningxia during different growth periods, the method of HPLC with switching wavelengths was used to analyze the contents of arctiin, arctigenin, and chlorogenic acid in different organs of cultivated A.lappa from Ningxia during different growth periods.Method: The HPLC analysis was carried out on Agilent C18 (4.6 mm × 250 mm, 5 μm) column at the temperature of 30 ℃;the mobile phase composed of acetonitrile and 0.1％ phosphoric acid with gradient elution at a flow rate of 1.0 mL · min-1, the detection wavelength was 280 nm shifted to 328 nm;the sample loading volume was 10 μL.Result: The contents of lignans and phenol chemicals were found to have larger variations in different organs and during different growth perioda;the fruit has the highest contents of the rctiin and arctigenin, and the content of chlorogenic acid is the highest in the leaves.Conclusion: This study has provided theoretical basis for determining the rational utilization and suitable harvest periods of arctium A-lappa, in Ningxia, as wellas for evaluating the product quality.
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万方数据
维普
