Optimization of Extraction Technology for Total Nucleosides from Codonopsis tangshen by Orthogomal Test
Objective:To optimize extraction conditions of total nucleosides in Codonopsis tangshen.Method:Ultrasonic method was adopted to extracting total nucleosides in C.tangshen.Taking the contents of total nucleosides (cytidine,uridine,guanosine and adenosine) as index,based on single factor tests,orthogonal test was designed to optimize extraction conditions of total nucleosides in C.tangshen.HPLC was employed to determine the contents of cytidine,uridine,guanosine and adenosine with mobile phase of methanol-water for gradient elution and detection wavelength at 260 nm.Result:The influence of various factors on the extraction rate of total nucleosides was on order of volume fraction of methanol ＞ extraction times ＞ solid-liquid ratio ＞ extraction time.Optimal parameters was as follows:extracted twice with 10 times the amount of water for 30 min of each time.Under these conditions,extracting amount of total nucleosides was 1.523 mg · g-1 Conclusion:This established HPLC method is accurate and reliable to detect contents of total nucleosides.At the same time,this optimized extraction technology is stable and feasible with the advantages of energy-saving,rapidity,efficient and so on.
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