Phenolic Compounds and Their Capacity of Scavenging Free Radicals from Residues in Pulps of Persea americana
Objective:To isolate and identify the chemical compounds from the residues in the pulps of Persea americana,and evaluate the capacity of scavenging free radicals of phenolic compounds.Method:The chemical compounds were isolated and purified by the combined normal pressure column chromatography and high pressure liquid preparative chromatography,and their chemical structures were elucidated by spectral data analysis and comparing with the previous literature.Moreover,the capacity of scavenging free radicals of phenolic compounds was also evaluated by using 1,1 diphenyl-2-trinitrobenzene hydrazine (DPPH ·) assay.Result:Seven compounds were obtained and their structures were identified as 1,2,4-trihydroxyheptadec-16-yne (1),1,2,4-trihydroxynonadecane (2),(2R,12Z,15Z)-2-hydroxy-4-oxoheneicosa-12,15-dien-l-yl acetate (3),tryptophan (4),chlorogenic acid (5),neochlorogenic acid (6),and scopolin (7) respectively.The in vitro free radicals scavenging activity experiment showed that the capacity of scavenging free radicals of compounds 5 and 6 were close to that of vitamin C.Conclusion:Compounds 4,6 and 7 were isolated from P.americana for the first time.In addition,phenolic compounds obtained from P.americana had good in vitro free radicalsscavenging activities.
Persea americanaresidues from the pulpschemical compoundscapacity of scavenging free radicals
NETL
NSTL
万方数据
维普
