首页|RP-HPLC同时测定不同产地路边青药材中4种活性成分的含量

RP-HPLC同时测定不同产地路边青药材中4种活性成分的含量

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目的:建立反相高效液相色谱法同时测定路边青药材中咖啡酸、槲皮素-3-O-葡萄糖苷、山柰酚-3-O-葡萄糖苷、山柰酚含量的方法.方法:采用Agilent C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.5％甲酸水溶液梯度洗脱,流速0.8 mL· min-1,柱温25℃,检测波长340 nm.结果:咖啡酸、槲皮素-3-O-葡萄糖苷、山柰酚-3-O-葡萄糖苷、山柰酚分别在0.045～1.130,0.095 ～2.370,0.105 ～2.635,0.041 ～1.030 μg线性关系良好,平均加样回收率(n=6)分别为99.0％,98.5％,97.7％,100.7％,RSD分别为1.0％,0.9％,1.0％,1.6％.结论:该方法简便、快捷,重复性好,可用于路边青药材的质量控制.
Simultaneous Determination of Four Constituents in Geum aleppicum by RP-HPLC
Objective:To establish a method for simultaneously determining caffeic acid,quercetin-3-O-glucoside,kaempferol-3-O-glucoside and kaempferol in Geum aleppicum by RP-HPLC.Method:The quantitative analysis of G.aleppicum was carried out on Agilent C18 column (4.6 mm ×250 mm,5 μm),mobile phase was acetonitrile-0.5％ formic acid with gradient elution,flow rate was 0.8 mL · min-1,columntemperature was maintained at 25 ℃,and detection wavelengrt was 340 nm.Result:The linear relationships of caffeic acid,quercetin-3-O-glucoside,kaempferol-3-O-glucoside and kaempferol fell within the ranges of 0.045-1.130,0.095-2.370,0.105-2.635,0.041-1.030 μg,respectively.Their average recoveries (n =6) were 99.0％,98.5％,97.7％,100.7％ with the relative standard deviations (RSDs) of 1.0％,0.9％,1.0％,1.6％,respectively.Conclusion:This method is simple,accurate,sensitive,which can be used for quality control of G.aleppicum.

Geum aleppicumcaffeic acidquercetin-3-O-glucosidekaempferol-3-O-glucosidekaempferol

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湖北省中西医结合医院,武汉 430015

路边青 咖啡酸 槲皮素-3-O-葡萄糖苷 山柰酚-3-O-葡萄糖苷 山柰酚

2016

10.13422/j.cnki.syfjx.2016140098

中国实验方剂学杂志
中国中医科学院中药研究所　中国中西医结合学会中药专业委员会

中国实验方剂学杂志

CSTPCDCSCD北大核心
影响因子：1.62
ISSN：1005-9903
年,卷(期)：2016.22(14)
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