Application of Vacuum Liquid Chromatography in Enrichment and Preparation of Active Ingredients of Traditional Chinese Medicines
With the development of traditional Chinese medicine (TCM) chemistry,more and more studies have focused on the separation and identification of micro-components and new components.However,their biological activity and development value have been hard to be studied due to the difficulties in enrichment and purification.Although,great progresses had been achieved in chromatography technology,such as HPLC and preparative HPLC,it is still hard to prepare in a large scale in laboratory.In order to find a suitable technique,vacuum liquid chromatography (VLC),which is an effective,convenient and cheap traditional separation technique,was recommended according to our experiences in reference examples during the preparation of these target compounds.Although VLC has been widely used in pharmaceutical,food and somatic industries,it was still a great challenge for new researchers in the field of separation and purification of TCM due to the lack of systematic disclosure of the technique.The characteristics,principle and key operating points of VLC and the separation strategy in laboratory were introduced in this paper to help study the separation and purification of TCM.
vacuum liquid chromatographyseparationtraditional Chinese medicine effective component
NETL
NSTL
万方数据
维普
