首页|不同产地牡丹皮中微量元素与多指标成分灰色关联度评价及相关性分析

不同产地牡丹皮中微量元素与多指标成分灰色关联度评价及相关性分析

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目的:测定不同产地不同加工方法牡丹皮药材中没食子酸、芍药内酯苷、芍药苷等7个成分及Fe,Mg等24种微量元素的含量,探讨牡丹皮药材中物质与微量元素间的关系,为牡丹皮药材的质量综合评价提供参考.方法:采用高效液相色谱波长融合方法测定没食子酸,芍药内酯苷,芍药苷,1,2,3,4,6-五没食子先葡萄糖,苯甲酸,苯甲酰芍药苷,丹皮酚7个成分的含量;采用微波消解-电感等离子体质谱法(ICP-MS)测定Fe,Mg等24种微量元素的含量;通过对指标成分、微量元素等进行相关分析并建立灰色关联度模型,综合评价不同产地不同加工方法牡丹皮的质量.结果:不同产地不同加工方法牡丹皮指标性成分及微量元素含量部分有显著差异(P<0.05),灰色关联度模型稳定,牡丹皮中的不同成分、各种微量元素之间存在显著的或极显著的正相关或负相关(P<0.05).结论:微量元素与多指标成分的相关性分析和灰色关联度模型为牡丹皮优质药材选择提供充分的依据和参考.
Grey Correlation Degree Evaluation and Correlation Analysis of Trace Elements and Active Ingredients in Moutan Cortex from Different Areas
Objective:To determinate the contents of seven components such as gallic acid,albiflorin and peoniflorin,and 24 trace elements in the medicinal plant Moutan Cortex from different areas,discuss the relationships between its various constituents,and provide a reference for the quality control of Moutan Cortex.Method:Microwave digestion-inductively coupled plasma mass spectrometry (ICP-MS) was employed to determine the contents of trace elements such as Fe and Mg;HPLC wavelength fusion method was used to determine the contents of gallic acid,albiflorin,paeoniflorin,1,2,3,4,6-penta-O-galloyl-beta-dglucopyranose,benzoic acid,benzoyl paeoniflorin,and paeonol.Grey correlation degree model and correlation analysis method were employed to comprehensively evaluate the quality of Moutan Cortex from the different areas and analyze the correlation between the contents of main components and trace elements.Result:There were significant differences in part principal components and the contents of trace elements in Moutan Cortex from different areas and processing methods (P < 0.05).The gray correlation degree model was stable,and there were significant or extremely significant positive correlation or negative correlation between different components and trace elements in Moutan Cortex (P < 0.05).Conclusion:The models of grey correlation degree and correlation analysis method can provide sufficient basis and reference for the selection of Moutan Cortex.

Moutan Cortextrace elementsactive componentsgrey correlation degreecorrelation analysis

刘威、王振中、胡军华、李家春、张振秋、萧伟

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辽宁中医药大学药学院,辽宁大连 116600

江苏康缘药业股份有限公司,江苏连云港222001

牡丹皮 微量元素 多指标成分 灰色关联 相关分析

国家“重大新药创制”科技重大专项

2013ZX09402203

2017

中国实验方剂学杂志
中国中医科学院中药研究所 中国中西医结合学会中药专业委员会

中国实验方剂学杂志

CSTPCDCSCD北大核心
影响因子:1.62
ISSN:1005-9903
年,卷(期):2017.23(1)
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