Quality of Phytolaccae Radix from Different Sources in Henan
Objective:To provide a basis for the development,utilization and qualitycontrol of Phytolacca Radix through quality and fingerprint analysis of Phytolacca Radix from different sources in Henan.Method:Thin-layer chromatography was used for qualitative identification;HPLC-ELSD method was used for the determination of esculentoside A content with moble phase of methanol-4% acetic acid (30:70);the flow rate was 1.0 mL· min-1 and column temperature was 30 ℃,evaporative light scattering detector (ELSD) was used for detection:gas flow rate of 2.0 L·min-1,drift tube temperature of 90 ℃,record time of 20 min and the sample size of 20 μL.Fingerprint analysis was conducted by using HPLC-ELSD and gradient elution,with a gas flow rate of 2.0 L·min-1,drift tube temperature of 105 ℃.Result:There was difference in content of esculentoside A of the samples from different areas,and the content was higher between 1.29%-3.52% in samples from Jiyuan,Xixia and Lushan;acid-insoluble ash content was qualified in all samples.There were 9 common peaks in the fingerprint,and peak 7 was the reference peak.Conclusion:The results of this study can be used as the reference for the formulation of Phytolaccae Radix quality standard.