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河南不同产地商陆药材质量分析

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目的:对河南不同产地商陆药材化学成分、酸不溶性灰分及指纹图谱进行分析,为商陆药材的开发利用、质量标准的制定提供参考依据.方法:采用薄层色谱法进行定性鉴定;HPLC-ELSD测定商陆皂苷甲含量,流动相甲醇-0.4%冰乙酸(30:70),流速1.0mL·min-1,柱温30℃,蒸发光散射检测器检测,气体流速2.0 L·min-1,漂移管温度90℃,记录时间20 min,进样量20μL.指纹图谱采用HPLC-ELSD法,梯度洗脱,气体流速2.0 L·min-1,漂移管温度105℃.结果:不同产地商陆中商陆皂苷甲含量存在差异,其中济源、西峡、鲁山产商陆中商陆皂苷甲的含量较高,在1.29% ~ 3.52%.酸不溶性灰分均合格.指纹图谱标出9个共有峰,以峰7(商陆皂苷甲)为参照峰.结论:该研究结果可作为制定商陆质量标准的参考依据.
Quality of Phytolaccae Radix from Different Sources in Henan
Objective:To provide a basis for the development,utilization and qualitycontrol of Phytolacca Radix through quality and fingerprint analysis of Phytolacca Radix from different sources in Henan.Method:Thin-layer chromatography was used for qualitative identification;HPLC-ELSD method was used for the determination of esculentoside A content with moble phase of methanol-4% acetic acid (30:70);the flow rate was 1.0 mL· min-1 and column temperature was 30 ℃,evaporative light scattering detector (ELSD) was used for detection:gas flow rate of 2.0 L·min-1,drift tube temperature of 90 ℃,record time of 20 min and the sample size of 20 μL.Fingerprint analysis was conducted by using HPLC-ELSD and gradient elution,with a gas flow rate of 2.0 L·min-1,drift tube temperature of 105 ℃.Result:There was difference in content of esculentoside A of the samples from different areas,and the content was higher between 1.29%-3.52% in samples from Jiyuan,Xixia and Lushan;acid-insoluble ash content was qualified in all samples.There were 9 common peaks in the fingerprint,and peak 7 was the reference peak.Conclusion:The results of this study can be used as the reference for the formulation of Phytolaccae Radix quality standard.

Phytolaccae Radixesculentoside AHPLC-ELSDHenan

裴莉昕、纪宝玉、陈随清、孙利平

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河南中医药大学药学院,郑州 450046

商陆药材 商陆皂苷甲 反相高效液相色谱-蒸发光散射检测法 河南产

中医药公共卫生专项资助项目中医药行业科研专项资助项目河南省中医药科学研究专项河南省高等学校重点科研项目

财社[2011]76号2012070022014ZY0204915A360008

2017

中国实验方剂学杂志
中国中医科学院中药研究所 中国中西医结合学会中药专业委员会

中国实验方剂学杂志

CSTPCDCSCD北大核心
影响因子:1.62
ISSN:1005-9903
年,卷(期):2017.23(2)
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