Effect of Huangqi Liuyi Tang on Glucose Re-absorption Via Renal Tubular Epithelial Cells in Type 2 Diabetes Model Rats
Objective:To observe the effect of Huangqi Liuyi Tang on diabetic rats' blood glucose,glycosylated hemoglobin,blood lipid and the renal tubular epithelial reabsorption of glucose transporter 2 (SGLT2),and explore its efficacy and possible mechanismt in the treatment of diabetes.Method:The 77 SPF rats were randomly divided into 6 groups by weight:normal control group,model group,metformin group (0.15 g·kg-1),Huangqi Liuyi Tang low,middle and high dose groups (3.15,6.30,12.60 g·kg-1).Diabetes models were established by using high fat and high glucose diet for 28 days and then with intraperitoneal injection of streptozotocin at the dose of 40 mg·kg-1 body weight.7 days after STZ injection,corresponding drugs were given by gavage.Equivalent volume of water was given in the normal control group and model control group by the same method.Gavage medication lasted for six weeks,once a week.Venous blood from the tails was collected to measure blood glycose after 6-hour fasting;after fasting 12-hour at the last time of gavage,the venous blood from the tails was taken for blood glycose and then the rats were anaesthetized to collect the abdominal venous blood.The kidney and pancreas were taken for histopathological examination after the rats were sacrificed.The blood glucose,glycosylated hemoglobin,blood lipid,body weight,food intake,and the expression of renal tissue SGLT2 were observed.Result:The blood glucose levels in modeling groups were higher than those in normal control group (P ＜0.01),with a blood glucose value ＞ 13.0 mmol · L-1.After six weeks self-healing,the blood glucose level in model group was still significantly higher than that of normal control group (P ＜ 0.01);the blood glucose levels in metformin group,Huangqi Liuyi Tang in middle and high dose groups were lower than that in model group (P ＜ 0.05).Glycosylated hemoglobin level was increased in model group and higher than that in the normal control group (P ＜ 0.01).As compared with the model group,glycosylated hemoglobin level was decreased in metformin group and the Huangqi Liuyi Tang high dose group (P ＜ 0.05).In the pancreas pathological HE staining in model rats,complete pancreas islet structure was seen,indicating that islet was not destroyed completely in this experiment.Renal pathological slices showed that the structures of renal glomerulus,collections,tubular,proximal and distal tubule were complete in all groups,with no obvious pathological damages.After treatment,SGLT2 protein expression levels were reduced in various treatment groups (P ＜ 0.05,P ＜ 0.01).Conclusion:High sugar and high fat diet with intraperitoneal injection of STZ increased SGLT2 protein expression in kidney tissues of diabetic models.Huangqi Liuyi Tang can reduce blood glucose,glycosylated hemoglobin,and lower SGLT2 protein expression in diabetes model rats.Its treatment efficacy for diabetes may be associated with partial inhibition of renal tubular epithelial reabsorption of glucose.
diabetesHuangqi Liuyi Tangblood glucoseglycosylated hemoglobinsodium-glucose transport protein-2 (SGLT2)
NETL
NSTL
万方数据
维普
