Pollen Viability and Stigma Receptivity of Polygala tenuifolia
Objective: To study the pollen viability and stigma receptivity of Polygala tenuifolia, so as to provide a scientific basis for sexual reproduction and breeding of new varieties. Method: The pollen viability of different blooming days (the day before flowering, day 1, day 2, day 3, day 4, day 5, day 6, day 7, day 8) were determined by the I2-KI method and the TTC method, and the staining effect of the two staining methods on pollen viability were compared. I2-KI method was used to determine the pollen activity of Polygala tenuifolia pollens in different periods of time, different storage methods (wet preserved flowers and dry preserved flowers) and different storage conditions (4, 26, -20, -80 ℃) in different periods of time (2, 4, 8, 24, 48, 72 h) . And its stigma receptivity was estimated by the benzidine-hydrogen peroxide. Result: (1) The pollen viability of P. tenuifolia decreased with the rise in the powder loosing time, the highest pollen viability was observed on the 4 thday after flowering; (2) from 8: 00 to 18: 00 during the daytime, the pollen viability between10: 00 and 16: 00 was stronger; (3) dry pollen can prolong the storage time under the condition of-20 ℃; (4) at the 5 thday after flowering, the stigma receptivity was the strongest. Conclusion: Pollen on the 4 thday after flowering can be collected, and artificial pollination can be adopted for stigma on the 5 thday after flowering stigma artificial pollination, so as to provide a theoretical basis for breeding of P. tenuifolia.
NETL
NSTL
万方数据
维普
