中药调控PI3K/Akt信号通路干预多囊卵巢综合征大鼠实验研究的现状、问题及对策
Current Situation,Problems and Countermeasures of Experimental Research on Traditional Chinese Medicine Regulating PI3K/Akt Signaling Pathway in Rats with Polycystic Ovary Syndrome
闫朋宣 1刘奕清 1咸楠星 1彭麟景 1李鲲 1张京春 2赵雨坤3
作者信息
- 1. 中国中医科学院,北京 100700
- 2. 中国中医科学院西苑医院,北京 100091
- 3. 中国中医科学院 中医基础理论研究所,北京 100700
- 折叠
摘要
多囊卵巢综合征(PCOS)及其导致的不孕症是妇科和生殖内分泌科常见的疾病之一.磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)信号通路是干预PCOS发展研究相对较多的信号通路,中药通过此信号通路开展的PCOS大鼠实验也是机制研究的主要方向.笔者通过相应标准梳理并筛选出了近5年在学术期刊公开发表的文章20篇,从基线资料、造模与治疗、分组情况、评价性指标、药效学指标5个方面对入选的研究客观情况、存在问题进行分析,结果发现各个研究项目均存在不同程度的问题,如造模观察指标、模型成功判断标准、治疗周期、方药/有效成分剂量计算方式和具体用量等未明确,易造成结果偏倚或降低实验数据有效度.基于此,形成了 PCOS大鼠实验研究操作清单,涉及实验大鼠、模型构建、研究实施、结局指标和分析与报告五大类共21条操作清单,以期为后续PCOS实验有关科研提供参考,助力形成高质量成果.
Abstract
Polycystic ovary syndrome(PCOS)and its resulting infertility is one of the common diseases of gynecology and reproductive endocrinology.The phosphatidylinositol 3-kinase/protein kinase B(PI3K/Akt)signaling pathway is relatively well-studied in the development of intervention in PCOS,and the experiments on PCOS in rats conducted by traditional Chinese medicine through this signaling pathway is also the main direction of mechanistic research.In this paper,20 articles published in academic journals in the past 5 years were selected through the corresponding criteria,and the objective situation and existing problems of the selected research projects were analyzed from five aspects,namely,baseline data,modeling and treatment,grouping,evaluative indexes,and pharmacodynamic indexes.It is found that there were different degrees of problems in each research project,such as the observation indicators of modeling,criteria for judging the success of the model,the treatment period,the calculation of dosage of prescription/active ingredients and specific dosage were not clearly defined,which could easily lead the bias of the results or reduce the validity of experimental data.Based on this,the list of PCOS rat experimental research operations was formed,involving five categories of experimental rats,model construction,study implementation,outcome measures and analysis and report with a total of 21 operation lists,with a view to provide a reference for the subsequent PCOS experiments related to scientific research and helping to form high-quality results.
关键词
中医药(TCM)/多囊卵巢综合征(PCOS)/磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)信号通路/现状及问题/动物模型/肾虚型/蛋白Key words
traditional Chinese medicine(TCM)/polycystic ovary syndrome(PCOS)/phosphatidylinositol 3-kinase/protein kinase B(PI3K/Akt)signaling pathway/current status and problems/animal model/kidney deficiency type/protein引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
维普
万方数据