Polygonati Odorati Rhizoma Polysaccharides Ameliorate Hyperlipidemia in Mice by Regulating Gut Microbiota
Objective:To explore the effect of Polygonati Odorati Rhizoma polysaccharides on hyperlipidemia in mice by modulating the gut microbiota.Methods:Ninety male C57BL/6J mice were randomized into the following groups(n=15):control,model,simvastatin,low-(100 mg·kg-1),medium-(200 mg·kg-1),and high-dose(400 mg·kg-1)Polygonati Odorati Rhizoma polysaccharides groups.Other groups except the control group were fed with a high-fat diet for the modeling of hyperlipidemia,and drug interventions lasted for 12 weeks.Serum levels of total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)were measured by an automatic biochemical analyzer.The pathological changes in the liver and epididymal fat were observed by hematoxylin-eosin staining,and lipid accumulation in the liver was assessed by oil red O staining.The gut microbiota was analyzed by 16S rRNA gene sequencing.Results:Compared with the control group,the model group exhibited an increase in body weight(P＜0.01),along with marked elevations in serum levels of TC,TG,and LDL-C(P＜0.05,P＜0.01).Furthermore,the model group showcased increase in the liver index and epididymal fat coefficient(P＜0.05),increased liver fat accumulation,enlargement of adipocytes in the epididymal fat,decreases in both alpha and beta diversity of the gut microbiota,and an increase in the relative abundance of Allobaculum(P＜0.01).Compared with the model group,Polygonati Odorati Rhizoma polysaccharides suppressed the increase in body weight(P＜0.01),lowered the serum levels of TC,TG,and LDL-C(P＜0.05,P＜0.01),reduced the liver index and epididymal fat coefficient(P＜0.05,alleviated liver fat accumulation,and decreased the size of adipocytes in the epididymal fat.Furthermore,it enhanced the alpha and beta diversity of the gut microbiota in mice,reduced the relative abundance of Allobaculum,Erysipelotrichaceae,and Clostridium(P＜0.01),and increased the relative abundance of Akkermansia and Blautia(P＜0.01).Conclusion:Polygonati Odorati Rhizoma polysaccharides can ameliorate hyperlipidemia induced by a high-fat diet in mice by regulating the diversity and composition of the gut microbiota.
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