Isolation of Porcine Epidemic Diarrhea Virus SD Strain and Development of in Vitro 3D Intestinal Organoids Infection Model
Porcine epidemic diarrhea virus(PEDV)is an intestinal coronavirus that infects the small intestines of piglets.The objective of the present study was to develop an in vitro 3D organoid model of porcine small intestines for studying the mechanism of PEDV infection.A PEDV SD strain was isolated from a farm and its whole genome sequence was analyzed in this study.The crypts of small intestines from a 10-day old pig were isolated and embedded in basement membrane extract and covered with the porcine intestinal organoids medium modified from the human system.The organoids with a crypt-villus architecture formed in 7 days.Then the swine organoids were infected with the PEDV SD strain virus.The PEDV infection was determined in indirect immunofluorescence assay.The results showed that the PEDV infection model of swine intestinal organoids were developed in this study.The 3D intestinal organoid model offered a long-term,renewable resource for investigating porcine intestinal infections with PEDV.
Porcine epidemic diarrhea virusswine intestinal organoidsinfection model
万方数据
维普
