Long Non-coding RNA lnc-AlVE1-AS1 Inhibits the Proliferation of A1V-J in Chicken Macrophages
Previous studies have shown that long non-coding RNAs derived from Endogenous retroviruses may be an important part of the antiviral immune response in macrophages.It has been reported that the long non-coding RNA lnc-ALVE1-AS1 derived from chicken Endogenous retrovirus is related to host genetic resistance and can activate the antiviral innate immunity of non-immune cells.In order to further explore the antiviral effect of lnc-ALVE1-AS1 in chicken macrophages and its mechanism,chicken macrophage cell line HD 11 was infected with ALV-J and lnc-ALVE1-ASl was found to be significantly down-regulated at 24 h as tested using the fluorescence quantitative PCR.Overexpression experiments further confirmed that lnc-ALVE1-AS1 significantly inhibited the proliferation of ALV-J in HD11 cells.The mechanism was reflected by the fact that lnc-ALVE1-AS1 significantly upregulated the expression of dsRNA recognition receptor(TLR3),type I interferons(IFN-α and IFN-β)and other antiviral innate immune genes(IRF7,MX1,OASL and IFITM3)in HD11 cells.However,the induction of type Ⅰ interferons(IFN-α and IFN-β)by lnc-ALVE1-AS1 was significantly reduced after TLR3 interference or TLR3 signals inhibition.Confocal localization analysis showed that lnc-ALVE1-AS1 directly bound to TLR3 protein in HD11 cells,suggesting that activation of TLR3 signals might be an important mechanism for lnc-ALVE1-AS1 to inhibit the proliferation of ALV-J in macrophages.This study revealed the antiviral function and mechanism of lnc-ALVE1-AS1 in macrophages and provided a new genetic basis for disease resistance breeding research.
万方数据
