Effect of Mycoplasma ovipneumoniae Infection on Transcript Levels of TLRs MyD88 Pathway Factors in Different Breeds of Sheep in Guizhou
Mycoplasma ovipneumoniae(Mo)can cause pneumonia in goats and sheep.The transcript levels of MyD88 pathway factor genes of Toll-like receptors(TLRs)was analyzed in the present study to determine the effect on susceptibility of Mo infection in different breeds of sheep and goats in Guizhou.Five major breeds of sheep and goats in Guizhou were experimentally infected with Mo and the transcript levels of TLRs MyD88 pathway factors in lung and blood samples were quantified and analyzed by using TaqMan RT-qPCR.The results showed that the relative mRNA expression of MyD88,TRAF6,NF-κB,FOS and JUN genes were all significantly up-regulated after Mo infection in different breeds of sheep and goats(P＜0.01).Meanwhile,the up-regulated transcript levels of MyD88 and FOS genes were significantly higher in the blood and lung samples of Guizhou black goats and Guizhou white goats that were infected and died than those of Boer goats and Lake sheep that were not infected and died(P＜0.05).In addition,the up-regulation of NF-κB gene transcript levels in the blood of infected non-dead Boer goats were all very significantly higher than in the remaining four species(P＜0.01).This difference in transcript levels might be due to the different pathogenesis of Mo in these breeds of sheep and goats.The above findings suggested that the susceptibility of different breeds of sheep and goats in Guizhou to Mo was related to the transcriptional levels of TLRs MyD88 pathway factors,providing basic research information for studying the pathogenic mechanisms of Mo in sheep and goats.
万方数据
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