In order to explore the effect of Eupatorium adenophorum on snail activity,snail tissues collected from Eupatorium adenophorum treated group,niclosamide-positive control group and blank control group were detected by ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometer(UPLC-Q-TOF/MS).Differential metabolites were screened by using principal component analysis(PCA),discriminant analysis of supervised partial least squares method(PLS-DA)and orthogonal partial least squares discriminant analysis(OPLS-DA).The results showed that the active ingredients of Eupatorium adenophorum interfered with the growth and metabolism of Oncomelania hupensis.A total of 79 different metabolites were screened,of which 31 metabolites such as glycine were significantly up-regulated and 48 metabolites such as 1-threonine were significantly down-regulated.In addition,18 metabolic pathways were revealed to clarify the intervening mechanisms of Eupatorium adenophorum for snail activity.These results laid a theoretical foundation for research and development of Eupatorium adenophorum and screening of molluscicides and molluscicidal drugs.
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