Comparison of the Immunization Effects of Six Kinds of Brucellosis Vaccines Worked on Sheep and Goats
In order to compare immunization effects of different Brucellosis vaccines worked on goats and sheep,385 sheep of native species aged 80-100 days and 275 Erlang Mountain goats aged 80-100 days were randomly selected among 6 towns of the Wulate grassland,they all tested negative of brucellosis,comparative immunization effect tests were carried out with six kinds of brucellosis vaccines.RBPT and SAT were adopted to detect the positive conversion rate and antibody titer respectively on 7,14,21,35,60,120,150,180,210,240 and 270 days of experimental group after vaccination.The challenge experiment was carried out 270 days after immunization.The results of RBPT and SAT showed that M5-90Δ26 and S2 were inoculated for sheep and goats obtained fast antibody production,and long duration;The results of the challenge test after immunization showed that the protection ratio of A19 dripped in sheep's eyes and the protection ratio of A19-ΔVirB12 with subcutaneous injection of sheep were all 4/6;The protection ratio of M5-90Δ26 with subcutaneous injection of sheep was 4/5,and ReV.1 dripped in goats'eyes got the same result in 4/5.The ultimate purpose of this experiment is to screen out the ideal vaccine for the establishment of cattle and sheep immunization-free area without brucellosis in the Wulate grassland,and to develop a reasonable immunization program and provide scientific basis for the creation of the country's first cattle and sheep immunization-free area without Brucellosis,at the same time,to give reference and guidance for the development,improvement and promotion of brucellosis vaccines and the prevention and treatment of Brucellosis in other regions of China.
万方数据
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