Development of an Indirect ELISA for Detection of Antibodies to PCV4 Cap Protein
In order to detect specific antibodies to Porcine circovirus type 4(PCV4)vaccine,an indirect ELISA method was developed in this study using PCV4 Cap protein as the coating antigen and PCV4 polyclonal rabbit antibodies as the primary antibodies and optimized for its reaction conditions.The optimal conditions included 2 μg/mL purified PCV4 Cap protein for coating plates overnight at 4℃,5％skimmed milk for blocking for 60 min,test serum dilution at 1:800 and reaction conditions at 37℃ for 45 min,conjugate dilution at 1:5000 and reaction conditions at 37℃ for 60 min,substrate development for 10 min,and cut-off value at 0.157.The sensitivity of this blocking ELISA was up to 102 400.The success of development of the blocking ELISA provided a precise and efficient method for detecting the specific antibodies induced by PCV4 vaccines.
Porcine circovirus type 4immuno detectionindirect ELISA
万方数据
维普
