Development of a TaqMan Probe Fluorescence Quantitative PCR Method for Detection of Nipah Virus
Nipah virus(NiV)infection is a severe infectious disease and also a zoonotic illness.In order to detect the virus quickly and specifically,specific primers and probe were designed in this study based on the conserved region of the NiV N gene.Subsequently,a TaqMan probe fluorescence quantitative PCR(qPCR)method was developed for detection of NiV.This qPCR method had good specificity without reaction with other swine viruses and high sensitivity with 10 times sensitive than conventional PCR and strong stability.In addition,its amplification efficiency reached to 102％and the minimum detection limit was 14.8 copies/μL.Availability of this qPCR method provided a technical means for the rapid detection of NiV and was of great significance to pig industry.
万方数据
维普
