A Study of Rapid Detection of Streptomycin-Resistant Mycobacterium tuberculosis with High-Resolution Melting Curves
The purpose of the present study was to explore the feasibility of high-resolution melting curve(HRM)technology in detecting streptomycin resistance of Mycobacterium tuberculosis using the traditional drug susceptibility test(DST).Meanwhile,the RpsL and the rrs genes consisting of RRDR was chosen to design primers for HRM analysis.SPSS17.0 software was used for statistical analysis and the coincidence of these two methods was calculated based on the results of DST.Kappa analysis was used to compare the difference and the consistency of the two methods.The results of DST showed that 20 strains were resistant to streptomycin and 64 strains were susceptible to streptomycin.HRM results showed that 24 strains were resistant to streptomycin,60 strains were susceptible to streptomycin.With the DST results by the proportion method as controls,the sensitivity and specificity of HRM in detection of streptomycin-resistance was 75％and 85.9％,respectively.The Kappa test showed moderate consistency between the two methods.The results of this study indicated that HRM quickly detected streptomycin resistance of M.tuberculosis with high sensitivity,which might be used in the early auxiliary diagnosis of drug-resistant tuberculosis.
万方数据
维普
