Detection and Complete Genomic Sequence Analysis of Porcine Epidemic Diarrhea Virus from a Diarrheal Pig Herd in Shanghai
Porcine epidemic diarrhea(PED)is a serious diarrheal disease of piglets caused by Porcine epidemic diarrhea virus(PEDV)infection.In October 2021,diarrhea occurred in piglets in the delivery room of a pig farm in Shanghai with the incidence rate of 30％-50％and mortality rate of 20％-30％.The pig herds had been vaccinated before.Fecal samples were examined by real-time RT-PCR for Porcine epidemic diarrhea virus,Transmissible gastroenteritis virus(TGEV),and Porcine rotavirus(RV).The results showed positive for PEDV and negative for TGEV and RV.Phylogenetic analysis of the whole genomic sequence showed that it belonged to the GⅡ-c subgroup and the S gene sequence belonged to the GⅡ-b subgroup.As a comparison,the whole genome and S gene of CV777 belonged to the GI-a subgroup,and the whole genome and S gene of AJ1102 belonged to the GⅡ-b subgroup.These results showed that a new genotype of PEDV had been prevalent in Shanghai and commercial vaccine strains did not provide complete protection due to genetic variation.
万方数据
维普
