Isolation and Identification of Two Marek's Disease Virus Strains and Sequence Analysis of Their Major Virulence Genes
In order to investigate genetic characteristics of Marek's disease virus(MDV)that prevailed in China,virus isolation was performed by inoculating anticoagulant blood samples of chickens with Marek's disease signs on cell cultures and the isolated viruses were identified in indirect immunofluorescent assay.meq and pp38 genes of the isolated MDVs were amplified by polymerase chain reaction.The obtained sequences were compared with that of the reference strains published in GenBank.The results showed that two MDV strains of serotype 1 were isolated and designated as JS0316 and JS0424.Comparison of the deduced amino acid sequence of meq gene showed that the molecular characteristics of two isolated MDV strains were inconsistent with that of the attenuated vaccine strain(A71S and P194-)but consistent with the molecular characteristics of virulent MDV isolates(D80Y,V115A,T139A,P176R and P217A).In addition,the strain JS0316,had mutations at Q93R and V123A in Meq protein and at L981 and F240S in pp38 protein.On the other hand,the strain JS0424 had mutations at P217T in Meq protein and W67G and V210A in pp38 protein.Therefore,these two MDV isolates obtained from this study possessed the molecular characteristics of virulent strains and developed new mutations.
Marek's disease virusisolation and identificationmeqpp38sequence analysis
国家科技期刊平台
NSTL
万方数据
维普
