Isolation and Characterization of Chinese Sacbrood Bee Virusand Experimental Infection in Larvae
The objectives of the present study were to investigate whether the bees on an apiary were infected with Chinese Sacbrood bee virus(CSBV)and to explore the infection features in Apis cerana.The bees suspected to be infected with CSBV were collected from the apiary and the presence of CSBV nucleic acid was examined by RT-PCR.The positive bee samples were collected for virus isolation and purification,electron microscope observation and sequencing.Three-day-old Apis cerana were experimentally infected with the purified and identified CSBV and transcriptome sequencing was performed to analyze the infection features of Apis cerana.As a result,a CSBV-JX strain was isolated and identified through RT-PCR and electron microscope observation.The diameter of CSBV-JX was observed by an electron microscope to be 25-30 nm.The CSBV-JX was amplified and sequenced in segments and the genome sequence was found to be 8781 bp.Phylogenetic analysis showed that the SBV of the Apis cerana(AcSBV)and the SBV of the Apis mellifera(AmSBV)were divided into two branches with solid regional difference.Transcriptome sequencing analysis revealed that 59 genes were up-regulated and 83 were down-regulated in Apis cerana at 2 hours post infection with CSBV-JX and while 68 genes were up-regulated and 86 genes were down-regulated in Apis cerana at 12 hours post infection with CSBV-JX.In summary,this study,the CSBV-JX strain isolated and identified and minor responses to experimental infection in named CSBV-JX.Apis cerana were demonstrated at 2 hours and 12 hours by transcriptomics analysis.The reason for minor infection might be at the early stages of virus multiplication.In early infection stages,the viral loads were low,causing minimal proliferation and replication.However,the differentially expressed genes(DEGs)were highly related to the metabolic pathways in Apis cerana at 2 hours and 12 hours post infection.In conclusion,the results of this study provided a basis for illustrating the molecular mechanism of CSBV infection in Apis cerana.
Chinese Sacbrood virusisolation and identificationphylogenetic analysistranscriptomics
国家科技期刊平台
NSTL
万方数据
维普
