Development and Application of a Real-Time PCR Method for Detection of Lumpy Skin Disease Virus
To develop a rapid diagnostic method for lumpy skin disease virus(LSDV),a real-time PCR method was developed using the primers and probe designed on the conserved sequence of GCPR gene.The standard curve of the real-time PCR assay had good linear relationships in the range of 3.67×101-3.67×107 copies/μL of template with the correlation coefficient to 0.9905.The assay had good sensitivity of 3.67×10 copies/μL.There was no cross-reaction with goat pox virus,bovine viral diarrhea virus,infectious rhinotracheitis virus,bovine parainfluenza virus type 3 and bovine respiratory syncytial virus.The assay was reproducible 0.133％to 1.81％of the coefficients of valuation within a batch and between batches.The agreement rate of this real-time PCR was 90％in testing clinical samples.In conclusion,the real-time PCR assay developed here could be used as a diagnostic tool for LSDV,thus provided a technical support for the epidemiological investigation of LSDV.
国家科技期刊平台
NSTL
万方数据
维普
