Establishment and preliminary application of detection methods for PCV2 and PCV3
Rapid detection and identification of PCV2 and PCV3.In this paper,probe primers were designed with reference to highly conserved fragments of PCV2 and PCV3 in GenBank,and a dual TaqMan fluorescence quantitative detection method was successfully established.The results show that this method has good specificity and can distinguish porcine circovirus type 2 and porcine circovirus type 3 from other porcine disease viruses.The minimum detection limit of PCV2 is 1.00×101 copies/μL,and that of PCV3 is 1.00×100 copies/μL;The repeatability is high,and the coefficient of variation between batches is 0.01％～0.02％and 0.02％～0.05％,respectively.In this study,440 pig samples with clinical symptoms such as reproductive disorder,panting,emaciation and pallor,skin inflammation were collected from large-scale pig farms in Hubei Province,and the established method and commercial kit were used for synchronous detection.The coincidence rate of PCV2 and PCV3 were 97.47％and 96.85％,respectively.The sequencing results showed that PCV2 was subtype 2d,and PVC3 was subtype 3c.The dual TaqMan fluorescence quantitative detection method was characterized by good specificity,sensitivity,rapidity,accuracy and cost saving.It can provide technical support for monitoring and prevention of PCV2 andPCV3.
PCV2PCV3Establishment of dual TaqMan qPCRmixed infection
国家科技期刊平台
NSTL
万方数据
维普
